Fig. 3. Variation of the enzymatic activity of GDH as a percent of the original value in the presence of various concentrations of octyl glucoside. The assays were carried out in 50 mM sodium phosphate buffer, pH 7.6, in the presence of 5 mM a-ketoglutarate, 50 mM NH₄Cl, 0.2 mM EDTA, and 0.1 mM NADH at 27°C. The enzyme concentration in the assay mixture was 0.36 nM.