Fig. 4. Variation of [Q]₂₂₂ for GDH (0.3 mg/ml) upon interaction with various concentrations of octyl glucoside (0-20 mM) in 50 mM sodium phosphate buffer, at pH 7.6. It can be seen that increasing the detergent concentration does not cause any significant alterations in the a-helix content of the protein in the concentration range of 0-20 mM octyl glucoside. Inset: Far-UV CD spectra of GDH (0.3 mg/ml) at various concentrations of octyl glucoside (0-20 mM) in 50 mM sodium phosphate buffer, pH 7.6. The inset shows no changes in the secondary structure upon treatment with various concentrations of octyl glucoside.