Fig. 6. The fluorescence emission spectra of: (a) ANS; (b) ANS + GDH; (c) ANS + GDH + 5 mM octyl glucoside; (d) ANS + GDH + 10 mM octyl glucoside; and (e) ANS + GDH + 20 mM octyl glucoside. The concentrations of ANS and GDH are 50 mM and 0.05 mg/ml, respectively. The excitation wavelength was 350 nm. All experiments were done in 50 mM sodium phosphate buffer, pH 7.6, at 27°C.