Fig. 7. Variation of fluorescence intensity with NADH concentration in the absence of octyl glucoside (a) and in the presence of 20 mM octyl glucoside (b). The fluorescence energy transfer studies were carried out by exciting the enzyme at 292 nm and recording NADH emission at the maximum emission value. The GDH concentration was 0.05 mg/ml. All experiments were done in 50 mM sodium phosphate buffer, pH 7.6 at 27°C.