Fig. 8. Double reciprocal plots of the reaction catalyzed by GDH using various concentrations of NADH in the presence of various concentrations of octyl glucoside: (a) 0 mM; (b) 5 mM; (c) 10 mM; (d) 15 mM; and (e) 20 mM. The assays were carried out in 50 mM sodium phosphate buffer, pH 7.6, in the presence of 5 mM a-ketoglutarate, 50 mM NH₄Cl, and 0.2 mM EDTA at 27°C. The enzyme concentration in the assay mixture was 0.36 nM.