Fig. 1. Effect of synthetic PG on ORC binding to origin DNA. ORC (0.15 pmol) was pre-incubated with the indicated amounts of CL, PG (egg yolk), PG (18:0) or PG (18:2) for 10 min at 30°C, and further incubated at 30°C for 10 min with radio-labeled ARS1 DNA fragments (90 fmol) in the presence (A) or absence (B) of 5 mg/ml poly dI/poly dC (A, B). A gel electrophoretic mobility shift assay was performed and the results subjected to autoradiography (A). The amount of bound DNA was determined by a filter-binding assay. Values are mean SD, n = 3 (B).