Fig. 2. Effect of synthetic PC on ORC binding to origin DNA. ORC (0.15 pmol) was pre-incubated with the indicated amounts of CL, PC (egg yolk), PC (18:0), or PC (18:2) for 10 min at 30°C, and further incubated at 30°C for 10 min with radio-labeled ARS1 DNA fragments (90 fmol) in the presence (A) or absence (B) of 5 g/ml poly dI/poly dC (A, B). A gel electrophoretic mobility shift assay was performed and the results subjected to autoradiography (A). The amount of bound DNA was determined by a filter-binding assay. Values are mean ± SD, n = 3 (B).