Fig. 4. Co-precipitation of ORC with phospholipids. ORC (0.15 pmol) was incubated with the indicated amounts of CL, PG (18:0), PG (18:2), PC (18:0), or PC (18:2) at 30°C for 5 min. After centrifugation, Orc1p in the precipitates was visualized by immunoblotting with anti-Orc1p antibodies (SB16 and SB35).